The system uses high intensity ultra-violet light. Do not remove the UV lamp while the system is running. Before replacing a UV lamp, ensure that the lamp cable is disconnected from the rear of the system to prevent injury to the eyes.
If the mercury lamp is broken, make sure that all mercury is removed and disposed according to national and local environmental regulations. Do not allow solutions that contain dissolved salts, proteins or other solid solutes to dry out in the flow cell.
Do not allow particles to enter the flow cell as damage to the flow cell may occur. This symbol indicates that the waste of electrical and electronic equipment must not be disposed as unsorted municipal waste and must be collected separately. Please contact an authorized representative of the manufacturer for information concerning the decommissioning of equipment.
Check for external or internal leakage. Run the Changing channel plate and distribution plate if required. A good laboratory routine is to calibrate the pH measurement once a day, when the electrode is replaced or if the ambient temperature changes. The pH electrode is calibrated using standard buffer solutions in a two point calibration. The two buffer solutions can have any pH value as long as the difference between them is at least 1 pH unit.
The calibration procedure can be done with the pH electrode either fitted in or removed from the flow cell. When calibrating the electrode out of the flow cell and changing from one buffer to another, rinse the electrode tip with distilled water and dab it carefully with a soft tissue to absorb the remaining water. Do NOT wipe the electrode as this may charge it and give unstable readings.
Press OK. Current calibration values are displayed buffer 1— buffer 2. The order of calibration, buffer 1 or buffer 2, is optional. Press OK to start with buffer 1, or press the down button to start with buffer 2.
In this example, we start with buffer 1. Then press OK. The asymmetry potential at pH 7 is shown as a mV value. Press Esc repeatedly to return to the Set Parameters menu. As the electrode ages, the slope decreases and the asymmetry potential increases. An electrode is still usable at lower slopes and higher asymmetry potentials but the response will be slower and the accuracy diminished. When calibrating with the electrode fitted in the flow cell, follow the above procedure but let at least 30—35 ml with 2 ml mixer of standard buffer solution be pumped through the system to stabilize pH.
Leave the pump running while calibrating. Switch to the second standard buffer solution and repeat the procedure. The relationship between pH and the output signal from the pH electrode is temperature dependent. For accurate measurements during temperature changes, the pH measurement can be temperature compensated.
In normal applications, when the temperatures of the buffers and calibration buffers are identical, temperature compensation is not necessary.
When using temperature compensation, it is important that the temperature of the pH electrode is the same as that of the conductivity flow cell since that is where the temperature is measured. The current setting for showing pH is displayed.
If on is shown, Tc is displayed in the running display. If off is shown default , Tc is not displayed. Press OK to change the setting. When necessary, replace the lamp assembly according to Changing the lamp assembly procedure optional , or contact your your local Cytiva service representative for lamp replacement. The autozero absorbance value for the wavelength used is shown.
The lamp and signal cables should be on the same side. As you slide the lamp housing into position, depress the two pressure pads on the filter housing in sequence to facilitate the installation. There will be a faint click when the housing is positioned correctly.
The Hg lamp housing can take up two positions, one for nm, marked by on the filter housing, and the other marked by for all other wavelengths. The Zn lamp housing has only one position. A click will indicate that the filter is in position. Replace it if it is not within limit. Pump a cleaning or sanitising agent through the flow cell. The standard recommendation is to pump 1 M NaOH for 30 minutes and then wash out with buffer. The tip of the pH electrode consists of a thin glass membrane.
Protect it from breakage, contamination and drying out or the electrode will be destroyed. Do NOT store in water only. Note: If the flow cell is full of liquid, it is not possible to insert the electrode.
If so, loosen the inlet connection while inserting the electrode to allow the liquid to run out from the flow cell. Remember to re-tighten the connector. Note: If the electrode is not fully inserted, the system will leak and a dead volume will occur in the holder. A spring is securing the chamber in position when the lock is opened. Replacement kits, Valve kit IV and Valve kit PV, are available see Spare parts recommended to keep on site in the Spare parts section for code no.
Loosen each one equally in turn so the distribution plate comes off parallel to the valve body. Using the Allen key, tighten the 4 screws in turn, a little at a time, until the distribution plate is fixed to the valve body.
If the conductivity measurements are not comparable to previous results, the electrodes in the flow cell may be contaminated and require cleaning.
To clean the flow cell:. Note: If the flow cell is totally blocked, the blockage can be removed using a needle or a wire with a diameter less than 0. If these procedures fail to rejuvenate the electrode, the problem is most likely a clogged liquid junction.
Faulty operation of the check valves is usually indicated by irregular flow, very low flow, or unstable pressure traces. Probable causes are air, dirt, or a damage in a check valve preventing it from closing to seal and hold the pressure.
Liquid appearing at a banjo fitting might indicate that a check valve O-ring is damaged. If this does not correct the problem, follow the instructions below to remove and then clean the valves. Before disassembling the pump, move the input liquid bottle below the level of the pump to prevent siphoning. It might also cause reduced flow or pressure fluctuation. Carefully replace both O-rings on the check valve with new ones if you suspect that an O-ring is damaged.
If cleaning of the check valve off-line does not correct the fault, replace the check valve with a new one. Note: Make sure that the check valves are installed in their correct positions. By mistake, an inlet check valve can be installed incorrectly in an outlet check valve position, and vice versa.
Note: Make sure that the inlet check valves are installed next to the inlet lower port on the connection part, and the outlet check valves next to the outlet upper port. Fasten the two attachment screws using the 4 mm hex wrench.
Please change the country on your profile in order to switch to another country store. Edit Profile. Product Support. In this section. Product Related FAQs. Thorough inspection and cleaning of system components Update of system firmware to ensure full compatibility of your system and UNICORN software Replacement of damaged or corroded seals, valve springs and solenoids Replacement of items that are reaching the end of their expected life — preventing future breakdowns Advice and guidance on proper daily use, cleaning and care of your system All work is documented and reported to help make any regulatory audits easier.
Which empty column are recommended for my system? If the measured flow rate differs the retention volumes need to be corrected. Run manually or make your own method. The measured retention volume is called Volume 1. Dismount the tubing from the UV flow cell and insert a low dead volume connector, e. The measured retention volume is called Volume 2. To do this a low dead volume connector, e. The valve is activated by setting Pump B inlet to the B2 position.
Method III - Determining the delay volume by balancing eluted water Manually set the flow path to the direction of the fraction collector. Unscrew the tubing that is connected to inlet of the UV flow cell and insert a luer adaptor instead. Fill a syringe with water and inject water into flow cell unless it drops at the outlet of the fraction collector in which case you have likely exceeded the pressure in the tubing which might be more than 4 bar, depending on configuration and flow restrictor used.
Now fill the syringe with air at least 20 ml because of compression and displace the water. Collect eluting water in a small cup. Determine the system delay volume by balancing the cup before and after elution. Repeat two times for calculation of a mean value.
Do not allow spilt liquid to dry on the instrument. Remove dirt from the surface using a cloth and a mild cleaning agent. Let the system dry completely before using it. When using hazardous chemicals, make sure that the entire system has been flushed thoroughly with bacteriostatic solution, e. NaOH, and distilled water before service and maintenance. Never leave the pH electrode in the electrode holder for any period of time when the system is not used, since this may cause the glass membrane of the electrode to dry out.
Do NOT store in water only! NaOH is injurious to health. Avoid spillage. Flow path schemes. Injection valve INV Something went wrong - we were not able to load your agreed MSA pricing. Please try refreshing the page. Spare parts. Spare parts, Accessory box delivered with the system. The kit contains:.
Spare parts, front view. Front view. Spare parts not indicated in the views. Spare parts for Injection valve INV Unions, ferrule and stop plug. Spare parts for pump P Spare parts for UV, pH and conductivity recording. Spare parts recommended to keep on site. Spare parts for Fraction collector. Spare parts for IV Mixer unusual appearance Issues related to Mixer unusual appearance Leakage.
Possible cause Suggested remedy Connector incorrectly fitted or worn Tighten or replace the connector if necessary - Check the mixer chamber. Buffer valve and injection valve unusual appearance Issues related to Buffer valve and Injection valve unusual appearance External leakage. Too low UV lamp intensity. Possible cause Suggested remedy The detector is not correctly fitted Check that the detector is fitted correctly. Dirty optical fiber Clean the connectors.
Dirt on optical sensors in detector Remove visible dirt on detector photo diodes. Worn-out or broken lamp Contact Service.
The UV module is not found by the instrument. Possible cause Suggested remedy Communication problem Contact Service. The internal temperature of the UV monitor is too high. No light transmission through the UV cell. Possible cause Suggested remedy Wrong wavelength for current buffer Change wavelength or buffer. Dirty optical fiber connectors Clean the connectors. Baseline drift or noisy signal. Possible cause Suggested remedy Flow restrictor in off-line position Use the flow restrictor.
Air in the UV flow cell Use the flow restrictor. Air in buffers De-gas if necessary. Impure buffers Check if the signal is noisy with water. If necessary, manually clean the UV cell. Autozero out of accepted range. Injection valve Issues related to injection valve The valve is not switching or is switching to wrong position. The Injection valve is not found by the instrument. Possible cause Suggested remedy Leaking connectors.
Conductivity monitor Issues related to Conductivity monitor Baseline drift of noisy signal. Possible cause Suggested remedy Air in the conductivity flow cell. Remove the air by flushing the flow cell with water or buffer. Leaking tubing connections Tighten the connectors. If necessary, replace the connectors. Dirty conductivity flow cell Clean the conductivity flow cell.
Possible cause Suggested remedy The conductivity monitor needs to be calibrated Check calibration with a solution with known conductivity. Calibrate the conductivity monitor. Dirty conductivity flow cell Clean the conductivity cell. Ghost peaks appear in the gradient profile.
Possible cause Suggested remedy Air bubbles are passing through the flow cell Check for loose tubing connections. A charged particle has been detected Prepare the sample so that charged particles are eliminated. Incorrect or unstable reading.
Possible cause Suggested remedy Poor mixing function Check that the correct mixer chamber size is used. The column is not equilibrated Equilibrate the column. The temperature compensation factor is not properly set Use a temperature compensation factor. Possible cause Suggested remedy The mixer chamber is too large Change to a mixer chamber with a smaller volume.
Bad pump function Make sure that the pump operates properly. Make sure that the tubing has been washed properly. Dirt in the tubing. Temperature out of range for calibration. Possible cause Suggested remedy This error could only occur when the temperature compensation is turned on. The cell constant measurement has been aborted. Possible cause Suggested remedy Internal errors See error log.
Unstable conductivity. Possible cause Suggested remedy Solids in the conductivity flow cell Clean the conductivity cell. Air in the conductivity flow cell Flush the conductivity flow cell with water. Waves on the gradient. Air in the flow path Purge the pumps. Poor mixing function Check that the correct mixer chamber size is used.
Inlet valves Issues related to inlet valves The inlet valves include Inlet valve A, Inlet valve B and sample inlet valve. The cable between the inlet valve and the ICU is not connected Remove the valve and make sure that the cable is connected. Faulty air sensor in the valve. Possible cause Suggested remedy Hardware error Restart the instrument with the power switch. Fraction collector Issues related to Fraction collector Too many commands pending in the fraction collector.
There is a risk that the tube is overfilled and fractionation movements lost. Possible cause Suggested remedy Too many commands are pending in the fraction collector The reason could be that too many fraction collector instructions have been sent. The tray is loose in the frac. Possible cause Suggested remedy The cassette tray positioning discs in the frac chamber are missing Replace the cassette tray positioning discs in the frac chamber.
The internal temperature of the fraction collector is too high. Possible cause Suggested remedy Hot surroundings Decrease the room temperature. The fraction collector failed to detect the code on the cassette. Dirty cassette type codes Clean the cassette type codes.
The fraction collector dispenser head failed to detect the flow properly and has switched to tube change with reduced accumulator functionality.
Possible cause Suggested remedy Air in the flow path Check the flow path for air. Dirty drop sync sensor diode windows Clean the drop sync sensor diode windows. The fraction collector dispenser head failed to detect a drop and has switched to tube change without Drop sync.
Possible cause Suggested remedy Too high flow rate Decrease the flow rate. Air in the flow path Check the flow path for air. The fraction collector dispenser head failed to detect a drop. Too high flow rate Decrease the flow rate.
The fraction collector cannot be found by the instrument. Possible cause Suggested remedy The cable between the fraction collector and the ICU is not connected Generate a System error report and contact Service.
The accumulator is jammed or there is an internal error in the instrument. Possible cause Suggested remedy Salt crystals or protein residuals block the accumulator. Restart the instrument and perform an accumulator wash. Mechanical error If this error is recurrent, generate a System error report and contact Service.
Fraction collector arm is blocked or internal fault in the fraction collector. Possible cause Suggested remedy Obstruction inside the fraction collector.
Calibration of the drop sync sensor failed. Possible cause Suggested remedy Dirty drop sync sensor diode windows Clean the drop sync sensor diode windows.
View more product information and purchase options. Disconnect the fingertight connector and the tube from the top of the UV flow cell, and replace the fingertight connector with a Luer connector. Disconnect the tube from the bottom of the UV flow cell, and connect a waste tube to the UV flow cell. Squirt the distilled water through the UV flow cell in small amounts. Inject the detergent solution remaining in the syringe into the flow cell. Inject the distilled water into the UV flow cell to rinse the flow cell.
Disconnect the Luer connector from the top of the UV flow cell, and reconnect the fingertight connector with the tube. Disconnect the waste tubing from the bottom of UV flow cell. Wipe the surface with a damp cloth. Lift off the Waste funnel and wash it with water and a mild cleaning agent.
Wipe off the Frac chamber using a damp cloth. In the System Control module, press the End icon in the toolbar. Open the System Control module and select System:Calibrate. Press the Prepare for calibration button. Fill a syringe with approximately 10 ml of chosen cleaning solution. If several cleaning solutions are to be used, repeat step 4 with distilled water and then with the next solution. Press the Close button. Result: The pH valve switches back to the default position and the Calibration dialog closes.
Disconnect the tubing from the pump head and disconnect the pump inlet tubing. Unscrew the purge valve by turning it counter-clockwise, and lift off the metal ring. Unscrew the plastic nut of the upper check valve using an adjustable wrench, and gently lift off the upper check valve. Unscrew the two white plastic screws located below each pump head. Unscrew the lower check valve using an adjustable wrench. Refit the check valves. Disconnect the fingertight connector and the tube from the top of the Conductivity monitor, and attach a Luer connector.
Disconnect the tube from the bottom of the Conductivity monitor, and connect a waste tube to the Conductivity monitor. Disconnect the Conductivity monitor from the rails, or slide the Conductivity monitor to the right on the rails. Fill a syringe with distilled water, and connect the syringe to the Luer connector. Squirt distilled water through the Conductivity flow cell in small amounts. Inject the distilled water into the Conductivity flow cell to rinse the flow cell.
Disconnect the Luer connector from the top of the Conductivity flow cell, and reconnect the fingertight connector with tubing. Acetonitrile Remarks : Quaternary valve not resistant. This method is more accurate than the one described above, but demands one more system. Manually set the flow path to the direction of the fraction collector.
Add to cart Get Quote. The cable between the Pressure monitors and the ICU is not connected. Air bubbles are passing through or are trapped in the pump. Replace the piston seal according to the instructions in Sections 7. Change the online filter in the mixer. The cable between the Column valve and the ICU is not connected. The cable between the outlet valve and the ICU is not connected. A dummy module is missing and the position is left empty.
Use the recommended mixer size for the used flow rate. The cable between the pH valve and the ICU is not connected. The temperature compensation of the pH monitor is turned off. Decreasing salt concentration in the electrode membrane due to osmosis to buffer. Replace the cell. Clean the connectors. Clean the UV cell, see Section 7. Replace UV flow cell. Perform a System CIP. The cable between the Injection valve and the ICU is not connected. Tighten the connectors. Clean the conductivity flow cell.
Check calibration with a solution with known conductivity. Clean the conductivity cell. Check for loose tubing connections. Prepare the sample so that charged particles are eliminated. Check that the correct mixer chamber size is used. The temperature compensation factor is not properly set. Purge the pumps. The cable between the inlet valve and the ICU is not connected. The cassette tray positioning discs in the frac chamber are missing.
Something went wrong - we were not able to load your agreed MSA pricing. Please try refreshing the page.
No reviews have been left for this product. Be the first to leave a review! Looking for support assistance? If you can't find what you need, contact us to solve your problems. Contact Us. Please change the country on your profile in order to switch to another country store.
Edit Profile. Something went wrong! Help us improve your experience by sending an error report. Thank you.
0コメント